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bis tris sds page acrylamide gels  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bis tris sds page acrylamide gels
    Bis Tris Sds Page Acrylamide Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bis+tris+sds+page/Acrylamide+%2F+N%2CN'-Methylenebisacrylamide+37%2E5%3A1%2C+for+biochemistry%2C+40%25+mix+solution+in+water/us12559749-860-22-26
    Average 94 stars, based on 1 article reviews
    bis tris sds page acrylamide gels - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Activity Assay:

    Article Title: Elucidating the activation mechanism of botulinum neurotoxin a: role of α-clostripain and NTNH
    Article Snippet: .. The activity of the activating protease in culture supernatant and culture supernatant fractions was determined by incubating the substrate LCH N (0.6 mg/mL) with the tested sample at 35 °C for 5 h. Thereafter, the enzymatic reactions were mixed with NovexTM Tris–glycine sample buffer (Thermo Fisher Scientific) supplemented with 5% ß-mercaptoethanol, heated (100 °C, 10 min) and loaded onto NuPAGETM 10%, Bis–Tris SDS-PAGE (Thermo Fisher Scientific). .. The gels were stained with InstantBlue® Coomassie Protein Stain (abcam).

    SDS Page:

    Article Title: Elucidating the activation mechanism of botulinum neurotoxin a: role of α-clostripain and NTNH
    Article Snippet: .. The activity of the activating protease in culture supernatant and culture supernatant fractions was determined by incubating the substrate LCH N (0.6 mg/mL) with the tested sample at 35 °C for 5 h. Thereafter, the enzymatic reactions were mixed with NovexTM Tris–glycine sample buffer (Thermo Fisher Scientific) supplemented with 5% ß-mercaptoethanol, heated (100 °C, 10 min) and loaded onto NuPAGETM 10%, Bis–Tris SDS-PAGE (Thermo Fisher Scientific). .. The gels were stained with InstantBlue® Coomassie Protein Stain (abcam).

    Article Title: Human Oncogene EWS::FLI1 Functions as a Pioneer Factor in Saccharomyces cerevisiae
    Article Snippet: .. Thawed samples were treated in 0.1 M NaOH for 15 minutes on ice, pelleted, resuspended in 100 μL of 2x LDS sample buffer, and boiled 10 min. Lysates were cleared by centrifugation at 10,000 x g and 10 μL were loaded in Bis-Tris SDS-PAGE (Bolt® 4-12% gradient; Invitrogen) and transferred to a nitrocellulose membrane. .. Membranes were blocked in 1.5% powdered milk in PBS-T during 1h 30min at RT, then incubated over-night at 4 °C primary antibodies: anti-EWSR1 (Santa Cruz Biotechnology, 1:500), and anti-GFP (Invitrogen, 1:500).

    Article Title: Bi-handed assembly chaperones regulate protein complex assembly through an intramolecular handover mechanism
    Article Snippet: .. Proteins were resolved on 8% bis-tris SDS-PAGE and detected either by Coomassie blue staining or, after transfer to PVDF membranes, by a reversible protein staining solution (Thermo Fisher Scientific, #YC369027) or by immunoblotting. ..

    Article Title: APP metabolism is regulated by p97/VCP through an autophagy and endolysosome-dependent mechanism
    Article Snippet: Images were captured using a Zeiss Axioplan Axiophot 2 Fluorescence Microscope. .. Cell homogenates were separated on 4-12% Bis-Tris SDS-PAGE (Invitrogen) or 16% Tris-Tricine gels (Bio-Rad) for APP-CTFs analysis. .. Proteins were transferred to either a 0.45 μm pore size nitrocellulose membrane (G&E Healthcare) or a 0.22 μm pore size nitrocellulose membrane for APP-CTFs analysis.

    Article Title: Engineering β-Galactosidase with Enhanced Catalytic and Transglycosylation Activity for GOS Production.
    Article Snippet: Lactases are important glycoside hydrolases that have widespread applications in the food and pharmaceutical industries.. However, the application of lactase is limited by its poor stability and transglycosylation activity.. In this study, a Kluyveromyces lactis-derived lactase was engineered to improve its hydrolytic activity and transglycosylation.

    Recombinant:

    Article Title: Phosphorylation enables progressive microtubule-associated protein proteolysis and functionalisation during neural development
    Article Snippet: .. Endogenous or recombinant protein samples were denatured by incubating with 3x LDS and RA buffer at 95°C for 5 minutes and then loaded directly on commercial NuPAGE 4–12% Bis–Tris SDS–PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) gels (Thermo Fisher Scientific). ..

    Nucleic Acid Electrophoresis:

    Article Title: Phosphorylation enables progressive microtubule-associated protein proteolysis and functionalisation during neural development
    Article Snippet: .. Endogenous or recombinant protein samples were denatured by incubating with 3x LDS and RA buffer at 95°C for 5 minutes and then loaded directly on commercial NuPAGE 4–12% Bis–Tris SDS–PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) gels (Thermo Fisher Scientific). ..

    Centrifugation:

    Article Title: Human Oncogene EWS::FLI1 Functions as a Pioneer Factor in Saccharomyces cerevisiae
    Article Snippet: .. Thawed samples were treated in 0.1 M NaOH for 15 minutes on ice, pelleted, resuspended in 100 μL of 2x LDS sample buffer, and boiled 10 min. Lysates were cleared by centrifugation at 10,000 x g and 10 μL were loaded in Bis-Tris SDS-PAGE (Bolt® 4-12% gradient; Invitrogen) and transferred to a nitrocellulose membrane. .. Membranes were blocked in 1.5% powdered milk in PBS-T during 1h 30min at RT, then incubated over-night at 4 °C primary antibodies: anti-EWSR1 (Santa Cruz Biotechnology, 1:500), and anti-GFP (Invitrogen, 1:500).

    Membrane:

    Article Title: Human Oncogene EWS::FLI1 Functions as a Pioneer Factor in Saccharomyces cerevisiae
    Article Snippet: .. Thawed samples were treated in 0.1 M NaOH for 15 minutes on ice, pelleted, resuspended in 100 μL of 2x LDS sample buffer, and boiled 10 min. Lysates were cleared by centrifugation at 10,000 x g and 10 μL were loaded in Bis-Tris SDS-PAGE (Bolt® 4-12% gradient; Invitrogen) and transferred to a nitrocellulose membrane. .. Membranes were blocked in 1.5% powdered milk in PBS-T during 1h 30min at RT, then incubated over-night at 4 °C primary antibodies: anti-EWSR1 (Santa Cruz Biotechnology, 1:500), and anti-GFP (Invitrogen, 1:500).

    Staining:

    Article Title: Bi-handed assembly chaperones regulate protein complex assembly through an intramolecular handover mechanism
    Article Snippet: .. Proteins were resolved on 8% bis-tris SDS-PAGE and detected either by Coomassie blue staining or, after transfer to PVDF membranes, by a reversible protein staining solution (Thermo Fisher Scientific, #YC369027) or by immunoblotting. ..

    Article Title: Transient silencing of spinal nociceptive neurons leads to a long-term reduction in joint pain mediated by recruitment of opioid activity
    Article Snippet: .. SDS-resistant and irreversibly assembled protein conjugates, SP-BOT, were visualised using Coomassie Blue stain in Novex NuPAGE 12% bis-tris SDS–PAGE (polyacrylamide gel electrophoresis) gels (Invitrogen) run at 4 °C in a NuPAGE MES SDS running buffer (Invitrogen). .. All recombinant proteins were expressed in the BL21-Gold (DE3)pLyss strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase C-terminal fusion proteins cleavable by thrombin.

    Article Title: Engineering β-Galactosidase with Enhanced Catalytic and Transglycosylation Activity for GOS Production.
    Article Snippet: Lactases are important glycoside hydrolases that have widespread applications in the food and pharmaceutical industries.. However, the application of lactase is limited by its poor stability and transglycosylation activity.. In this study, a Kluyveromyces lactis-derived lactase was engineered to improve its hydrolytic activity and transglycosylation.

    Western Blot:

    Article Title: Bi-handed assembly chaperones regulate protein complex assembly through an intramolecular handover mechanism
    Article Snippet: .. Proteins were resolved on 8% bis-tris SDS-PAGE and detected either by Coomassie blue staining or, after transfer to PVDF membranes, by a reversible protein staining solution (Thermo Fisher Scientific, #YC369027) or by immunoblotting. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Transient silencing of spinal nociceptive neurons leads to a long-term reduction in joint pain mediated by recruitment of opioid activity
    Article Snippet: .. SDS-resistant and irreversibly assembled protein conjugates, SP-BOT, were visualised using Coomassie Blue stain in Novex NuPAGE 12% bis-tris SDS–PAGE (polyacrylamide gel electrophoresis) gels (Invitrogen) run at 4 °C in a NuPAGE MES SDS running buffer (Invitrogen). .. All recombinant proteins were expressed in the BL21-Gold (DE3)pLyss strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase C-terminal fusion proteins cleavable by thrombin.



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    Thermo Fisher bis tris sds page
    (A) SY5Y-APP695 cells were transfected with scrambled siRNA (siScr) or VCP siRNA (siVCP) for 72 hours. <t>(A)</t> <t>SDS-PAGE</t> resolved cell protein lysates, and transferred membranes were blotted with antibodies targeting VCP, poly-ubiquitinated proteins (Poly-Ub), SQSTM1 (p62), APP-Cter-C17 (APP), and β-actin as a protein loading control. Apparent molecular weights are indicated to the left of the Western blots in kilodaltons (kDa) (B and C). Densitometric analyses and semi-quantification of VCP, Poly-Ub proteins, immature (mi.) and mature (mat.) APP levels, and APP α-carboxy-terminal fragments (APP-αCTF). (D) The concentrations of Aβ 1-40 and Aβ 1-42 peptides were measured in the cell-conditioned media by ELISA. Histograms represent the mean ± SD on the y-axis and are expressed as a percentage of the control value, arbitrarily set at 100%. n=6 *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 Unpaired t-test.
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    Thermo Fisher bis tris sds page gels
    (A) SY5Y-APP695 cells were transfected with scrambled siRNA (siScr) or VCP siRNA (siVCP) for 72 hours. <t>(A)</t> <t>SDS-PAGE</t> resolved cell protein lysates, and transferred membranes were blotted with antibodies targeting VCP, poly-ubiquitinated proteins (Poly-Ub), SQSTM1 (p62), APP-Cter-C17 (APP), and β-actin as a protein loading control. Apparent molecular weights are indicated to the left of the Western blots in kilodaltons (kDa) (B and C). Densitometric analyses and semi-quantification of VCP, Poly-Ub proteins, immature (mi.) and mature (mat.) APP levels, and APP α-carboxy-terminal fragments (APP-αCTF). (D) The concentrations of Aβ 1-40 and Aβ 1-42 peptides were measured in the cell-conditioned media by ELISA. Histograms represent the mean ± SD on the y-axis and are expressed as a percentage of the control value, arbitrarily set at 100%. n=6 *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 Unpaired t-test.
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    Image Search Results


    (A) SY5Y-APP695 cells were transfected with scrambled siRNA (siScr) or VCP siRNA (siVCP) for 72 hours. (A) SDS-PAGE resolved cell protein lysates, and transferred membranes were blotted with antibodies targeting VCP, poly-ubiquitinated proteins (Poly-Ub), SQSTM1 (p62), APP-Cter-C17 (APP), and β-actin as a protein loading control. Apparent molecular weights are indicated to the left of the Western blots in kilodaltons (kDa) (B and C). Densitometric analyses and semi-quantification of VCP, Poly-Ub proteins, immature (mi.) and mature (mat.) APP levels, and APP α-carboxy-terminal fragments (APP-αCTF). (D) The concentrations of Aβ 1-40 and Aβ 1-42 peptides were measured in the cell-conditioned media by ELISA. Histograms represent the mean ± SD on the y-axis and are expressed as a percentage of the control value, arbitrarily set at 100%. n=6 *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 Unpaired t-test.

    Journal: bioRxiv

    Article Title: APP metabolism is regulated by p97/VCP through an autophagy and endolysosome-dependent mechanism

    doi: 10.64898/2026.02.03.703492

    Figure Lengend Snippet: (A) SY5Y-APP695 cells were transfected with scrambled siRNA (siScr) or VCP siRNA (siVCP) for 72 hours. (A) SDS-PAGE resolved cell protein lysates, and transferred membranes were blotted with antibodies targeting VCP, poly-ubiquitinated proteins (Poly-Ub), SQSTM1 (p62), APP-Cter-C17 (APP), and β-actin as a protein loading control. Apparent molecular weights are indicated to the left of the Western blots in kilodaltons (kDa) (B and C). Densitometric analyses and semi-quantification of VCP, Poly-Ub proteins, immature (mi.) and mature (mat.) APP levels, and APP α-carboxy-terminal fragments (APP-αCTF). (D) The concentrations of Aβ 1-40 and Aβ 1-42 peptides were measured in the cell-conditioned media by ELISA. Histograms represent the mean ± SD on the y-axis and are expressed as a percentage of the control value, arbitrarily set at 100%. n=6 *p<0.05, **p<0.01, ***p<0.005, ****p<0.001 Unpaired t-test.

    Article Snippet: Cell homogenates were separated on 4-12% Bis-Tris SDS-PAGE (Invitrogen) or 16% Tris-Tricine gels (Bio-Rad) for APP-CTFs analysis.

    Techniques: Transfection, SDS Page, Control, Western Blot, Enzyme-linked Immunosorbent Assay

    (A) SY5Y-APP695 cells were transfected with negative control scrambled siRNA (siVCP -) or with VCP siRNA (siVCP +) for 72h and then treated with 40µg/mL of cycloheximide (CHX) for the indicated times from 0 to 3 hrs. Western membranes were immunoblotted with the following antibodies: APP-Cter-C17 (APP im. and APP mat.), VCP, and β-actin as a loading control. (B and C) Densitometric analyses and quantification of immature and mature APP levels were expressed as a percentage of the control (Ctrl) on the y-axis, over CHX treatment time on the x-axis. The black dotted lines represent quantification for the scrambled siRNA, while the grey dotted lines represent the siVCP condition (D). VCP siRNA reduces cell surface APP levels in SY5Y-APP695 cells. SY5Y-APP695 cells were transfected with scrambled siRNA (-) or with VCP siRNA (+) for 72h. Cell-surface proteins were chemically biotinylated and isolated with immobilized avidin beads. Flow-through and avidin-bound proteins were resolved by SDS-PAGE, and APP, VCP, and Calnexin (a cytosolic protein) were detected using antibodies against APP (APP Cter-C17), VCP, and calnexin. (E) Densitometric analysis and quantification of total APP levels. The graph indicates the mean ± SD. n=4 *p<0.05, **p<0.0, Unpaired t-test.

    Journal: bioRxiv

    Article Title: APP metabolism is regulated by p97/VCP through an autophagy and endolysosome-dependent mechanism

    doi: 10.64898/2026.02.03.703492

    Figure Lengend Snippet: (A) SY5Y-APP695 cells were transfected with negative control scrambled siRNA (siVCP -) or with VCP siRNA (siVCP +) for 72h and then treated with 40µg/mL of cycloheximide (CHX) for the indicated times from 0 to 3 hrs. Western membranes were immunoblotted with the following antibodies: APP-Cter-C17 (APP im. and APP mat.), VCP, and β-actin as a loading control. (B and C) Densitometric analyses and quantification of immature and mature APP levels were expressed as a percentage of the control (Ctrl) on the y-axis, over CHX treatment time on the x-axis. The black dotted lines represent quantification for the scrambled siRNA, while the grey dotted lines represent the siVCP condition (D). VCP siRNA reduces cell surface APP levels in SY5Y-APP695 cells. SY5Y-APP695 cells were transfected with scrambled siRNA (-) or with VCP siRNA (+) for 72h. Cell-surface proteins were chemically biotinylated and isolated with immobilized avidin beads. Flow-through and avidin-bound proteins were resolved by SDS-PAGE, and APP, VCP, and Calnexin (a cytosolic protein) were detected using antibodies against APP (APP Cter-C17), VCP, and calnexin. (E) Densitometric analysis and quantification of total APP levels. The graph indicates the mean ± SD. n=4 *p<0.05, **p<0.0, Unpaired t-test.

    Article Snippet: Cell homogenates were separated on 4-12% Bis-Tris SDS-PAGE (Invitrogen) or 16% Tris-Tricine gels (Bio-Rad) for APP-CTFs analysis.

    Techniques: Transfection, Negative Control, Western Blot, Control, Isolation, Avidin-Biotin Assay, SDS Page

    (A) VCP colocalizes with APP in SY5Y-C99 cells, as shown by co-labeling with APP-Cter-C17 (Red) and VCP (Green) antibodies. (B) VCP was immunoprecipitated, and C99 was detected only following C99-induced expression (C99 lane) compared to the non-induced condition (Ctrl lane). (C) SY5Y-C99 cells were treated for 24 hours with 1.5 µM of the allosteric VCP ATPase inhibitor NMS-873 as indicated. (C-D) SDS-PAGE-resolved cell protein lysates and nitrocellulose membranes were immunolabeled with the following antibodies: VCP, APP-Cter-C17, and Histone H3 as a loading control. (E) ELISA analysis of conditioned medium from SY5Y-C99 cells was performed to quantify secreted Aβ 1-40 and Aβ 1-42 . Histograms show the mean ± SD. n=3; ns=non-significant, *p<0.05, **p<0.01, ***p<0.001. Data were analyzed using an unpaired t-test or Mann-Whitney test.

    Journal: bioRxiv

    Article Title: APP metabolism is regulated by p97/VCP through an autophagy and endolysosome-dependent mechanism

    doi: 10.64898/2026.02.03.703492

    Figure Lengend Snippet: (A) VCP colocalizes with APP in SY5Y-C99 cells, as shown by co-labeling with APP-Cter-C17 (Red) and VCP (Green) antibodies. (B) VCP was immunoprecipitated, and C99 was detected only following C99-induced expression (C99 lane) compared to the non-induced condition (Ctrl lane). (C) SY5Y-C99 cells were treated for 24 hours with 1.5 µM of the allosteric VCP ATPase inhibitor NMS-873 as indicated. (C-D) SDS-PAGE-resolved cell protein lysates and nitrocellulose membranes were immunolabeled with the following antibodies: VCP, APP-Cter-C17, and Histone H3 as a loading control. (E) ELISA analysis of conditioned medium from SY5Y-C99 cells was performed to quantify secreted Aβ 1-40 and Aβ 1-42 . Histograms show the mean ± SD. n=3; ns=non-significant, *p<0.05, **p<0.01, ***p<0.001. Data were analyzed using an unpaired t-test or Mann-Whitney test.

    Article Snippet: Cell homogenates were separated on 4-12% Bis-Tris SDS-PAGE (Invitrogen) or 16% Tris-Tricine gels (Bio-Rad) for APP-CTFs analysis.

    Techniques: Labeling, Immunoprecipitation, Expressing, SDS Page, Immunolabeling, Control, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    (A) SY5Y-APP695 cells were treated for 24 h with autophagy activator Torin1 (1µM), autophagy flux inhibitors Bafilomycin A1 (BafA1, 100nM) or Chloroquine (CQ, 30 µM). Protein cell lysates were resolved by SDS-PAGE, transferred, and nitrocellulose membranes were blotted with APP-Cter-C17 rabbit polyclonal serum for the detection of mature (Mat.) and immature (im.) APP as well as APP carboxy-terminal fragments (APP-βCTF and APP-αCTF). β-actin staining was used as a protein loading control. Apparent molecular weight in kilodaltons (kDa) using molecular weight markers is indicated to the left of the Western blot images. Mature and immature proteoforms of APP were quantified (B) as well as α- and β-CTF (C) and Aβ 1-40 and Aβ 1-42 peptides (D). (E) SY5Y-APP695 cells were treated for 24 h with NMS-873 alone or NMS-873 together with the autophagy activator Torin1 (1µM), autophagy flux inhibitors Bafilomycin A1 (BafA1, 100nM) or Chloroquine (CQ, 30 µM). Following SDS-PAGE, mature and immature proteoforms of APP and APP-CTFs were detected with APP-Cter-C17 polysera and β-actin, used as a protein loading control. Immature and mature APP proteoforms were quantified (F) as well as APP-CTFs (G) and A β1-40 and 1-42 peptides (H). Data are represented as histograms of the mean ± SD (minimum of n = 3 independent experiments produced in triplicate for each experimental condition), ns = non-significant, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Journal: bioRxiv

    Article Title: APP metabolism is regulated by p97/VCP through an autophagy and endolysosome-dependent mechanism

    doi: 10.64898/2026.02.03.703492

    Figure Lengend Snippet: (A) SY5Y-APP695 cells were treated for 24 h with autophagy activator Torin1 (1µM), autophagy flux inhibitors Bafilomycin A1 (BafA1, 100nM) or Chloroquine (CQ, 30 µM). Protein cell lysates were resolved by SDS-PAGE, transferred, and nitrocellulose membranes were blotted with APP-Cter-C17 rabbit polyclonal serum for the detection of mature (Mat.) and immature (im.) APP as well as APP carboxy-terminal fragments (APP-βCTF and APP-αCTF). β-actin staining was used as a protein loading control. Apparent molecular weight in kilodaltons (kDa) using molecular weight markers is indicated to the left of the Western blot images. Mature and immature proteoforms of APP were quantified (B) as well as α- and β-CTF (C) and Aβ 1-40 and Aβ 1-42 peptides (D). (E) SY5Y-APP695 cells were treated for 24 h with NMS-873 alone or NMS-873 together with the autophagy activator Torin1 (1µM), autophagy flux inhibitors Bafilomycin A1 (BafA1, 100nM) or Chloroquine (CQ, 30 µM). Following SDS-PAGE, mature and immature proteoforms of APP and APP-CTFs were detected with APP-Cter-C17 polysera and β-actin, used as a protein loading control. Immature and mature APP proteoforms were quantified (F) as well as APP-CTFs (G) and A β1-40 and 1-42 peptides (H). Data are represented as histograms of the mean ± SD (minimum of n = 3 independent experiments produced in triplicate for each experimental condition), ns = non-significant, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Article Snippet: Cell homogenates were separated on 4-12% Bis-Tris SDS-PAGE (Invitrogen) or 16% Tris-Tricine gels (Bio-Rad) for APP-CTFs analysis.

    Techniques: SDS Page, Staining, Control, Molecular Weight, Western Blot, Produced